Excised Patch Chamber with Two Slotted Open Baths (Warner)

This chamber is designed for electrophysiological recordings of excised patches. The dual bath construction permits exchange of the solution surrounding the membrane patch without contamination of the entire population. The chamber consists of two independent bath compartments separated by a dam/reservoir which functions as a gate and barrier to prevent mixture of the solutions. The composition and exchange rate of each bath can be controlled independently.

After patching onto a cell in the larger bath compartment, the dam/reservoir is flooded with solution establishing a "solution bridge" between the two compartments. This enables secure transfer of the electrode attached cell to the smaller compartment. The dam/reservoir is then emptied by vacuum suction thus separating the two baths.

This model is used with either P-6 or PH-6 (heater) platform.



Dimensions, small bath  (L × W × H)
    20.0 × 3.8 × 4.5 mm

Dimensions, large bath  (L × W × H)
    28.0 × 9.5 × 4.5 mm

Volume, small bath, by depth
    111 µl

Volume, large bath, by depth
    243 µl

Specimen area, small bath (L × W)
    28 × 3.8 mm

Specimen area, large bath (L × W)
    21 × 9.5 mm

Bottom coverglass
    24 × 50 mm

Input tubing  (ID × OD)
    1.14 × 1.57 mm (PE-160)

Aspirator coupling
    1.67 mm (OD)